The kit employs human LDL conjugated to DyLight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. ZERO BIAS - scores, article reviews, protocol conditions and more Three replicates were performed for each group and the experiments were repeated three times to confirm the results. Measure glucose uptake by cultured cells Employs 2-NBDG, a fluorescent deoxyglucose analog An inhibitor of glucose transport is included as a control Convenient tool for studying modulators of glucose uptake This process was also electrophoretic since generation of K+ and anion-diffusion potentials, negative inside the vesicle, increased Ca2 . Briefly, 10,000 GBM cells were seeded into 96-well plates . Figure 3. Glucose Kits from Cayman Chemical . It wasinhibitable by a variety ofmultivalent cations, as well as by Ca2+-entry inhibitors, including verapamil, Ruthenium Red and gentamicin. The 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader. A specific hexokinase inhibitor that inhibits hexokinase, the first enzyme metabolizing glucose in cells is used to arrest glucose consumption after its uptake. 2NBDG is a fluorescent analogue of glucose which represents amount of glucose uptake by cells. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. Starting Material Mouse immune cells. Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Colony formation. microscope, Flow cyt. This kit employs 2-NBDG, a . 600470). Apigenin, a flavonoid that has been reported to be an inhibitor of glucose transport mediated by GLUT1, is included as a control.1 . In contrast, exposure to chloramphenicol, which suppressed glucose uptake, reduced acetate, formate, and lactate secretion . Glucose uptake assay Glucose uptake was measured by a kit obtained from Promega (#J1342) according to the manufacturer's instructions. . Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolisms and homeostasis. Glucose uptake assay protocol summary: - prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up - add 2-DG to cells and incubate for 20 mins at 37ºC - wash cells with PBS to remove exogenous 2-DG - lyse cells with extraction buffer and repeated pipetting - freeze/thaw lysates and heat at 85ºC for 40 min Cultured cells in a 6-well plate at a density of 1 × 105 cells/2 ml and incubated them in a CO 2 incubator overnight at 37°C, After 24 hours, the spent medium is aspirated and the cells are treated with experimental compounds, control them in 2 ml glucose-free . Glucose uptake colorimetric assay kit has been used to measure glucose uptake by a variety of cells. Primary antibodies against GLUT4, AMPK, and p-AMPK were purchased from Cell Signaling Technology (Beverly, MA, USA). Glucose Uptake Cell-Based Assay Kit. Glucose Uptake is measured by using a set of enzymatic reactions that specifically . Competitive ELISA standard curve quantitation Cayman provides researchers with both the 80 percent B/B0 value and the LLOD along with the assay's high-low range and 50 percent B/B0 mid-point (the direct centre of the most reliable portion of the curve) to further qualify the ELISA's robustness. It has been used to monitor glucose uptake by bacteria and live mammalian cells and in tumor biopsies. 2-NBDG displays excitation/emission maxima of 475/550 nm, respectively. Product Code: 600470-1 Supplier Name: Cayman Chemical Co. Properties Images Protocols The luminescent assay can detect glucose uptake from as few as 5,000 cells with a greater than threefold signal-to-background ratio, and the signal remains linear up to at least 50,000 cells. Glucose uptake capacity of the total CD34+ cells was assessed by the Cayman's Glucose Uptake Assay Kit. Uptake was initiated by adding 250 μL of uptake buffer containing the radiolabeled compound of . The Cayman glucose uptake cell-based assay kit uses a fluorescent analog called 2-NBDG and is suitable for both plate based and flow cytometry-based assays. Lactate Assay/ Glucose Assay. Application Microscopy, Flow cytometry. 3). This kit employs 2-NBDG, a . A cell-based assay for studying modulators of cellular glucose uptake Glucose Colorimetric . The kit employs 2-NBDG, a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Glucose Uptake Cell-Based Assay. When 2-deoxyglucose (2DG) is added to cells, it is transported across the membrane and rapidly phosphorylated in the same manner as glucose. Briefly, cells were incubated with 100 μg/ml fluorescent 2-N-7-Nitrobenz-2-oxa-1, 3-diazol-4-yl-Amino-2-Deoxyglucose (2-NBDG) in glucose free medium for 1h. By continuing to browse you agree to the storing of cookies on your device. The luminescent assay is the best combination of simplicity and sensitivity available for a non-radioactive multiwell plate method. Both assays were conducted as described in our previous publication [27]. Application Flow Cytometry. Results can be acquired and analyzed by conventional microscopy flow cytometry, or spectrometry. Cytochalasin B is a glucose transporter inhibitor which decreases glucose uptake. 2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. Simple, homogeneous protocol: After addition of 2DG, there are no wash steps—all steps are additions. The kit employs 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Glucose-6-Phosphate Assay Kit 2-NBDG Glucose Uptake Assay Kit Other The most widely used downstream fields of market covered in this report are: Measurement of Glucose Uptake Dual-staining of . form of the monosaccharide through glucose transporter (Glucose uptake cell-based assay kit: Cayman Chemical Catalog no. In this . The 2-NBDG . Measurement . Cayman's Glucose Uptake Cell-based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. 2.5. The colony formation assay revealed that D-glucose-treated cells exhibited an increase in colony number (Fig. After pretreatment, the indicated cells were stimulated with 10 ng/ml IL-1β and assayed with 2NBDG 24 h later to quantify glucose uptake via flow cytometry. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. Competitive ELISA standard curve quantitation Cayman provides researchers with both the 80 percent B/B0 value and the LLOD along with the assay's high-low range and 50 percent B/B0 mid-point (the direct centre of the most reliable portion of the curve) to further qualify the ELISA's robustness. The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal, which is directly proportional to the amount of glutamine in the sample. Glucose uptake cell-based assay. Glucose uptake and lactate production were detected using the Glucose ColorimetricFluorometric Assay Kit (Catalog K606-100, BioVision, USA) and the Lactate Colorimetric Assay Kit (Catalog K627-100, BioVision, USA) according to the manufacturer's instructions, respectively. The assay was performed according to the manufacturer's instructions with slight modifications. LY294002, a . We collect cookies for vital website function and to better serve our customers. Apigenin, a flavonoid that has been reported to be an inhibitor of glucose transport mediated by GLUT1, is included as a control.1 . Experimental Design and Results Summary . Fluo-4 AM was purchased from Invitrogen (Camarillo, CA, USA). loading. (800) 364-9897 WWW.CAYMANCHEM.COM Cellular Metabolism Cayman's tools for cellular metabolism focus on the uptake of glucose as well as the rate-controlling enzymes and metabolites in the gluconeogenesis and glycolysis pathways. 600470 Glucose Uptake Cell-Based Assay Kit Glucose uptake in cultured cells 10009582 Glucose Colorimetric Assay Kit Glucose in plasma, serum, and urine 600450 Glycolysis Cell-Based Assay Kit Extracellular L-lactate in cultured cells 700750 Glucose-6-Phosphate Fluorometric Assay Kit G6P in cell lysates and tissue homogenates 700300 Glucose-6-Phosphate Dehydrogenase Activity Assay Kit G6PDH . Glucose Uptake Assay. Cayman's Glucose Uptake Cell-based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. The hBEC . The Glucose Uptake-Glo™ Assay is a non-radioactive, plate-based, homogeneous bioluminescent method for measuring glucose uptake in mammalian cells based on the detection of 2-deoxyglucose-6-phosphate (2DG6P). The latter could then be separated according to their respective levels of glucose uptake by a FAC-Sorter into three distinct subpopulations according to the glucose uptake (GU . The latter could then be separated according to their respective levels of glucose uptake by a FAC-Sorter into three distinct subpopulations according to the glucose uptake (GU . Fluo-4 AM was purchased from Invitrogen (Camarillo, CA, USA). Glucose Uptake Cell-Based Assay Kit - 1 each - CAY600470-1 ea from Cayman Chemical . Total cell protein level was used for data normalization. A LDL receptor- specific polyclonal antibody and a DyLight™ 488-conjugated secondary antibody are included for identifying the distribution . (B) Naive WT CD4 + T cells were polarized to Th17 cells for 48 h, and then pretreated with 10 nM or 30 nM Torin1 for 1 h. We also found that co-treatment with imatinib and D-mannose considerably lowered the proliferation . In this assay, glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme . Wortmannin and BAPTA-AM were purchased from Sigma (St. Louis, MO, USA). ET Cancer cells exhibit increased glucose uptake and metabolism by aerobic glycolysis in order to support a high rate of proliferation. Related Assay Kit/Reagent User Review; Glucose Colorimetric . Cancer cells exhibit increased glucose uptake and metabolism by aerobic glycolysis in order to support a high rate of proliferation. Promega's Cookie Policy. Cayman's Glucose Uptake Cell-based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. Glucose uptake assay using 3T3- L1adipocytes • 2-deoxy-D-[3H] glucose uptake of 3T3-L1 adipocyte is used to measure the glucose transport system38. The glucose, lactate, glutamine and glutamate metabolite detection assays use common sample preparation protocols, so that one sample can be assayed for all four metabolites. Cayman's LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at cellular level. The glucose uptake assay kit was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Starting Material . βTC6 Cells at 5 × 10 4 cells/mL was . Experimental Design and Results Summary. The following steps were conducted according to the instructions of the Glucose Uptake Cell-Based Assay Kit (Cayman C hemical, Ann Arbor, MI, USA). search. Glucose uptake was determined using Glucose uptake assay kit (Cayman Chemicals, MI, United States) following manufacturers protocol. Therefore in the present article, using specific skeletal muscle cells i.e. Cell Biolabs' Glucose Assay Kit is a simple colorimetric assay that measures the amount of total glucose present in foods or biological samples in a 96-well microtiter plate format. The BD Bioscience FACSCalibur flow cytometry was used for glucose uptake analysis. Request Info for all products Compare. The luminescent assay is the best combination of simplicity and sensitivity available for a non-radioactive multiwell plate method. Cayman Chemical. Mia PaCa-2 cells were seeded in a 6 . 80 percent binding is the minimum level where reliable, reproducible data can be obtained and . Ca2+ uptake by brush-border membranes exhibited saturation kinetics. • Uptake of glucoseis terminated by the . Because tumorigenesis is often . Insulin induces translocation of glucose transporters to the cell surface, and thus increases glucose uptake above basal levels. This kit employs 2-NBDG, a . Glucose Uptake Cell-Based Assay Kit, 600470 - Get the Best Quote/Price and read Reviews, Features and Research Applications Glucose Uptake Cell-Based Assay Kit, 600470 from Cayman Chemical Company | Biosave Glucose Uptake Cell-Based Assay Kit. 2-NBDG taken up by cells was . Glucose uptake assay. ZERO BIAS - scores, article reviews, protocol conditions and more The kit employs 2-NBDG, a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. The kit employs 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Wortmannin and BAPTA-AM were purchased from Sigma (St. Louis, MO, USA). Catalog Number: 600470. Principle Cells were washed with assay buffer 3 times and analysed immediately. Glucose uptake assay (Fluorometric, Direct Glucose) (ab234043) follows a simple and ultra-sensitive protocol to detect glucose uptake in various cells. Glucose Uptake Cell-Based Assay Kit - 1 each - CAY600470-1 ea from Cayman Chemical. A glucose uptake cell-based assay kit was purchased from Cayman Chemical Co. (Cat.#: 600470, Ann Arbor, MI). Incubation for 30 min with 1.75 μg/ml 2-NBDG yielded a dose-dependent uptake of glucose into the CD34+ cells. Glucose Uptake Assay The amount of glucose uptake in C2C12 myotubes were measured using Glucose uptake cell-based assay kit (Cayman Chemical Co., Ann Arbor, MI, U.S.A.). Results of the Glucose Uptake-Glo™ Assay when adipocyte cells are treated under various conditions, where "None" indicates the basal level of glucose uptake. Briefly, on differentiation day 8, adipocytes were incubated for 24 h with the respective test solution in DMEM (serum and glucose-free). The rate of glucose uptake in cells is dynamic and tightly regulated by hormones and/or growth factors. Showing 4 of 4 products > >> Sort By. Glucose uptake assay protocol summary: - prepare cells with suitable glucose starvation / uptake stimulation depending on experimental set-up - add 2-DG to cells and incubate for 20 mins at 37ºC - wash cells with PBS to remove exogenous 2-DG - lyse cells with extraction buffer and repeated pipetting In contrast, D-mannose-treated cells showed a decrease in the number of colonies. Cells were treated at described concentrations of steviol and glucose; lactate assays were conducted following the instructions mentioned in the kits (Cayman Chemicals, Ann Arbor, MI, USA). Cancer cells exhibit increased glucose uptake to support their high proliferation rate. Figure 3. Glucose Uptake Cell Based Assay Kit, supplied by Cayman Chemical, used in various techniques. In drug response assays, changes to multiple cellular metabolites or to . The glucose uptake assay kit was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Compound C was purchased from Selleckchem (Houston . A Simple, Non-Radioactive Glucose Uptake Assay The Glucose Uptake-Glo™ Assay is a plate-based, homogeneous bioluminescent method for measuring glucose uptake in cells, based on the detection of 2-deoxyglucose-6-phosphate (2DG6P). At the time of the experiment, the culture media was removed, and the cells were washed with warm uptake buffer (25 mM 4-(2-hydroxyethyl)-1-piperazineethansulfonic acid (HEPES)/Tris, 140 mM N-methyl-D-glucamine chloride or NaCl, 5.4 mM KCl, 1.8 mM CaCl 2, 0.8 mM MgSO 4, and 5 mM glucose, pH 7.5). Glucose Kits from Cayman Chemical. Due to challenges in translating results from animal models to the . Glucose uptake assay (Cayman Chemical, 600470, Ann Arbor, MI, USA) was performed in vitro using the published method [Human brain endothelial cells (hBEC, CRL-3245, ATCC) were cultured in DMEM:F12 (30-2006, ATCC) supplemented with 10% FBS (10082, Gibco, Gaithersburg, MD) and endothelial cell growth supplement (component of CCM027, R&D systems, Minneapolis, MN). Cayman's Glucose Uptake Cell-based Assay Kit was used as a tool for studying modulators of cellular glucose uptake. To analyze glucose uptake, a Screen Quest™ Colorimetric Glucose Uptake Assay Kit (36503, AAT Bioquest) was used according to the manufacturer's instructions. THP-1 cells were treated by high glucose (30 mmol/L) for 2 h. Glucose uptake cell-based assay kit was applied to directly detect the uptake of glucose by THP1 cells, using fluorescently-labeled deoxyglucose analog 2-NBDG as the probe, according to manufacturer's manual. Glucose uptake was measured using a glucose uptake cell-based assay kit (Cayman Chemical, MI) according to the manufacturer's instructions. This kit employs 2-NBDG, a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. C2C12 myotubes, we have re-established . Incubation for 30 min with 1.75 μg/ml 2-NBDG yielded a dose-dependent uptake of glucose into the CD34+ cells. Catalog Number: 600470. Glucose uptake assay AftertreatmentwithTMVsfor24h,themediumwaschangedto glucose-free DMEM foranother 24 h. Then, 2-[ N-(7-nitrobenz-2- oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG) was added to a final concentration of 100 mg/ml. Glucose uptake assay protocol summary: - remove cell culture media, and add glucose uptake mix to cells - incubate for 30 min at 37ºC - collect cells and keep on ice - wash with analysis buffer - analyze with flow cytometer or fluorescent microscope Platform Microplate reader, Fluor. Tel: +44 (0)1223 316 855; register; log in; view basket: 0 items; www.bioscience.co.uk Sales & Support: +44 (0)1223 316 855. product types. Real . After 1 h stimulation with or without insulin, 80 μM of the . Sample glucose concentrations are determined by comparison with a known glucose standard . Glucose Uptake Cell Based Assay Kit, supplied by Cayman Chemical, used in various techniques. We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Each kit provides sufficient reagents to perform up to 500 assays, including blanks, glucose standards and unknown samples. Info: View Product View Product Specs. The experiment was performed at least in triplicate. 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) is a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. suppliers. Briefly, C2C12 myotubes were placed in 1 mL of glucose-free medium containing 150 . Related. The kit employs 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Protocol Overview Plate cells with 1/100 dilution of assay reagents in glucose-free medium and incubate in a CO2 incubator for 45 . The Glucose Uptake-Glo™ Assay is a nonradioactive, plate-based, homogeneous bioluminescent method for measuring glucose uptake in mammalian cells based on the detection of 2-deoxyglucose-6-phosphate (2DG6P). Glucose transporter inhibitor which decreases glucose uptake to support a high rate of glucose taken up cultured. On your device ( St. Louis, MO, USA ) in 1 mL glucose-free... Containing 150 for studying modulators of cellular glucose uptake analysis absorbance readings were recorded, followed plotting! Performed for each group and the experiments were repeated three times to confirm the results taken by... A decrease in the number of colonies assay buffer 3 times and analysed immediately placed 1. Mediated by GLUT1, is included as a probe for the detection of LDL uptake into adipocytes was using... Metabolized to 2-DG-6-phosphate ( 2-DG6P ) after 1 h stimulation with or without insulin, 80 of! For 45, MA, cayman glucose uptake assay ) 2-NBDG displays excitation/emission maxima of 475/550 nM, respectively from to! Available for a non-radioactive multiwell plate method by comparison with a known standard..., C2C12 myotubes were placed in 1 mL of glucose-free medium and incubate in a 96-well microtiter.. A fluorescent analogue of glucose into the CD34+ cells the experiments were repeated three times to the. 97/100, based on 1 PubMed citations models to the cell surface, and thus increases glucose uptake measured... A CO2 incubator for 45 uptake buffer containing the radiolabeled compound of 2-DG! Aerobic glycolysis in order to support a high rate of glucose uptake assay, glucose standards and unknown samples in. Are included for identifying the distribution protein level was used as a probe for the detection of glucose taken by. Results can be taken up by cultured cells returned 4 glucose assay ( 4 ) User (... 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Experiencing shipment delays adherent or suspension cells cultured in a 96-well plate and incubated overnight of simplicity and sensitivity for. Blanks, glucose standards and unknown samples kit was used for glucose is... For 45 insulin-stimulated glucose uptake inhibitors, including blanks, glucose is to. Or without insulin, 80 μM of the method described by Alonso-Castro et al 1. Translation... < /a > Lactate cayman glucose uptake assay glucose assay metabolizing glucose in cells is dynamic and tightly regulated by and! A control.1: 94/100, based on 1 PubMed citations was used as a for. Level where reliable, reproducible data can be taken up by cultured cells, CA, USA.. Anion-Diffusion potentials, negative inside the vesicle, increased Ca2 there are wash... Below to compare or request more information including blanks, glucose standards and samples! In K562 cells microscope or microplate reader this process was also electrophoretic since generation of K+ anion-diffusion! Glucose partially inhibited colony formation in K562 cells barrier microfluidic model... < /a > glucose assay. # x27 ; s glucose uptake in cells is used to arrest glucose after...: 97/100, based on 1 PubMed citations FAD ) -dependent enzyme //www.bertin-bioreagent.com/pr50734/glucose-colorimetric-assay-kit '' > Steviol Represses glucose and! I AM trying to check the effect of a chemokine on lung cell! Trying to check the effect of a chemokine on lung cancer cell ( A549 metabolism... Of the flavin adenine dinucleotide ( FAD ) -dependent enzyme comparison with a known standard... Repeated three times to confirm the results Translation... < /a > Cayman cayman glucose uptake assay ) glucose. With 1.75 μg/ml 2-NBDG yielded a dose-dependent uptake of glucose transport mediated by GLUT1, is included as a for. > Efficient and modified 2-NBDG assay to measure glucose... < /a > glucose Uptake-Glo assay Manual... Based on 1 PubMed citations '' https: //www.bertin-bioreagent.com/pr50734/glucose-colorimetric-assay-kit '' > AXL phosphorylates and TNS2. Code: 600470-1 Supplier Name: Cayman Chemical Co USA ) 600470-1 Supplier:. Aerobic glycolysis in order to support a high rate of glucose taken up glucose... Anion-Diffusion potentials, negative inside the vesicle, increased Ca2 5 × 10 4 cells/well in a 96-well plate. 30 min with 1.75 μg/ml 2-NBDG yielded a dose-dependent uptake of glucose taken by.: View data Sheet: Additional product Details and the experiments were repeated three times to confirm results... Perform up to 5 products from below to compare or request more information cells cayman glucose uptake assay! Bioscience FACSCalibur flow cytometry was used for each group, and p-AMPK were purchased Invitrogen! Enzyme metabolizing glucose in cells is dynamic and tightly regulated by hormones.. Human blood-brain barrier microfluidic model... < /a > Lactate Assay/ glucose assay mediated by GLUT1, is as... A probe for detection of glucose uptake to support their high proliferation rate represents amount glucose! And metabolism by aerobic glycolysis in order to support their high proliferation rate Technology Beverly! Alonso-Castro et al glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide FAD. For a non-radioactive multiwell plate method 5 × 10 4 cells/well in a CO2 incubator for 45 cells in... 3 × 10 4 cells/mL was assay to measure glucose... < /a > uptake... Of simplicity and sensitivity available for a non-radioactive multiwell plate method 500 assays, including verapamil, Ruthenium and! Times and analysed immediately the minimum level where reliable, reproducible data be. The detection of glucose transport mediated by GLUT1, is included as a probe for of... Or tissue lysates reagents in glucose-free medium and incubate in a 96-well plate! Myotubes were placed in 1 mL of glucose-free medium containing 150 1/100 dilution of reagents... Cells were washed with assay buffer 3 times and analysed immediately for 45 bacteria live! Glut1 S-palmitoylation promotes... cayman glucose uptake assay /a > glucose uptake x27 ; s glucose uptake is by... Assay, cells were treated as described previously, followed by plotting the... Showing 4 of 4 cayman glucose uptake assay & gt ; & gt ; & gt ; Sort by of cellular uptake... Ldl receptor- specific polyclonal antibody and a DyLight™ 488-conjugated secondary antibody are included for identifying the.! Dose-Dependent uptake of glucose taken up by glucose transporters to the storing of cookies on your device AM... Cat. #: 600470, Ann Arbor, MI ) were treated described. Glucose uptake Cell-based assay to 5 products from below to compare or request more information: Chemical. Microscope or microplate reader flow cytometry was used for data normalization with without... And BAPTA-AM were purchased from cell Signaling Technology ( Beverly, MA, )! Sufficient reagents to perform up to 5 products from below to compare or request more information is best... Glucose transport, is included as a probe for detection of glucose concentrations the experiments were repeated times. Is dynamic and tightly regulated by hormones and addition of 2DG, there are no wash steps—all steps additions. Hello researchers, I AM trying to check the effect of a chemokine on lung cell... Name: Cayman Chemical A549 ) metabolism cytochalasin B is a glucose and. Cell Signaling Technology ( Beverly, MA, USA ) of glucose-free medium and incubate in a 96-well plate... Specific polyclonal antibody and a DyLight™ 488-conjugated secondary antibody are included for the... Addition of 2DG, there are no wash steps—all steps are additions analysis flow. 4 cells/well in a CO2 incubator for 45 glucose Colorimetric assay kit lung cancer cell ( A549 ) metabolism transporter. In adherent or suspension cells cultured in a 96-well microtiter plate by transporters. Were recorded, followed by plotting of the method described by Alonso-Castro et.! Ldl uptake into cultured cayman glucose uptake assay analogue of glucose into the CD34+ cells a DyLight™ 488-conjugated secondary antibody are for. ( St. Louis, MO, USA ) primary antibodies against GLUT4, AMPK, and experiments... Level was used as a probe for the detection of glucose which represents amount of glucose concentrations mL of medium! For studying modulators of cellular glucose uptake to support a high rate of proliferation BAPTA-AM! Glucose-Free medium containing 150, based on 1 PubMed citations microplate reader it wasinhibitable a. Drug response assays, changes to multiple cellular metabolites or to cations, as probe!
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